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Joint Research Group Macromolecular Crystallography

Cell Culture & Expression Lab – Supporting Protein Production at the HZB BioLab

The Cell Culture & Expression Lab at the HZB-MX BioLab supports the production of proteins in prokaryotic and eukaryotic cell cultures, from transformation to cell harvesting and lysis. As part of the S1 laboratory facility, this space provides essential equipment for researchers working on protein expression and sample preparation for structural biology, biochemistry, and X-ray diffraction studies.

Clean Bench NuAire Nu 440-400E
Incubator Shakers Innova 43R andThermo Fischer MaxQ

Capabilities & Workflow

  • Transformation & Cell Cultivation – Supports bacterial and yeast cell culture workflows.
  • Protein Expression – Provides optimized conditions for small- to medium-scale protein production in various expression systems.
  • Cell Harvesting & Lysis – Equipped for efficient cell disruption and sample processing.

Key Equipment

  • Clean Bench (NuAire Nu 440-400E) – Provides a sterile workspace for cell handling.
  • CO₂-Incubator (Nunc) – For prokaryotic & eukaryotic cell culture, with or without CO₂.
  • Incubator Shakers (Innova 43R & Thermo Fischer MaxQ) – Temperature-controlled bacterial and yeast culture growth.
  • Centrifuge (Avanti J-E, Beckman Coulter) – For high-speed cell harvesting.
  • Ultrasonic Homogenizer (Bandelin Sonopuls) – For efficient cell lysis and protein extraction.
  • -20°C Freezer – For sample and reagent storage.

Access & Support

The Culture & Expression Lab is available to internal and external users as part of the HZB user platform. Researchers can access the facility and receive guidance on protein expression, troubleshooting, and sample preparation techniques.

For more information, please contact us.