New sample holder for protein crystallography
Up to three indivudal drops may be placed onto the sample holder. © HZB
24 sample holders are grouped. © HZB
After their formation the tiny crystals are prepared for x-ray analysis - without touching them. They stay onto the same sample holder. © HZB
An HZB research team has developed a novel sample holder that considerably facilitates the preparation of protein crystals for structural analysis. A short video by the team shows how proteins in solution can be crystallised directly onto the new sample holders themselves, then analysed using the MX beamlines at BESSY II. A patent has already been granted and a manufacturer found.
Proteins are huge molecules that often have complex three-dimensional structure and morphology that can include side chains, folds, and twists. This three-dimensional shape is often the determining factor of their function in organisms. It is therefore important to understand the structure of proteins both for fundamental research in biology and for the development of new drugs. To accomplish this, proteins are first precipitated from solution as tiny crystals, then analysed using facilities such as the MX beamlines at BESSY II in order to generate a computer image of the macromolecular structure from the data.
Up to now, protein crystals have first been grown and then transferred onto a sample holder for structural analysis. However, this transfer entailed a risk of destroying the often extremely fragile crystals.
This risk is no longer necessary thanks to the new sample holder developed by Dr. Manfred Weiss and Dr. Christian Feiler from the MX team together with Dr. Dirk Wallacher from the BESSY II sample environment group. Instead, the protein solution is applied directly onto the sample holder and crystallised in place, eliminating the need to transfer the delicate protein crystals to a different sample holder for analysis. “The new sample holder saves work steps and reduces the risk of damaging the sensitive protein crystals“, explains Feiler. “We have a short video clip that shows step-by-step how these sample holders facilitate protein crystallography – you have to see this!” exclaims Weiss, head of the MX-Beamline.
In practice, a large number of samples are always measured at once, so 24 sample holders are grouped together onto one sample plate. The new sample holder is patented in Germany and registered for an international patent. Jena Bioscience has acquired a licence and is already marketing the new development worldwide.
Published in J. Vis. Exp. (2019): An All-in-one Sample Holder for Macromolecular X-ray Crystallography with Minimal Background Scattering. Christian G. Feiler, Dirk Wallacher, Manfred S. Weiss
Video and publication
- A New Era in Catalysis: ASCEND Launch in Berlin, €30 Million in FundingOn 11 June 2026, the Helmholtz-Zentrum Berlin (HZB) in Adlershof hosted the launch of ASCEND (Accelerated Solutions for Catalysis using Emerging Nanotechnology and Digital Innovation). The event took place in the presence of the Minister of Research, Dorothee Bär, President of the Helmholtz Association, Prof. Dr. Martin Keller, and President of the Max Planck Society, Prof. Dr. Patrick Cramer. Bringing together leading partners from industry and research, ASCEND is supported by BMFTR with €30 million in funding and officially started on 1 April 2026. The initiative aims to accelerate the discovery of next-generation catalysts and enable more sustainable chemical processes.
- Magnon momentum microscopy: A new window into nanoscale spin-wavesAn international team lead by the Max Born Institute has developed a new type of momentum microscopy to image magnons — the quanta of collectively excited spins — directly in two-dimensional reciprocal space using soft X-rays. Measurements have taken place at BESSY II and PETRA III, first author ist the HZB physicist Steffen Wittrock. Owing to its remarkable sensitivity, simplicity, and access to nanometer-scale wavelengths, this novel technique establishes a powerful and versatile platform for exploring nonlinear magnon interactions, which are promising for future computing schemes.
- Imaging Ellipsometry for Process Control of Thin-Film DevicesA German–Israeli research team led by Dr. Andreas Furchner has demonstrated how imaging ellipsometry enables non-destructive characterisation and quality control of microstructured MXene thin films during device fabrication. The authors used two complementary ellipsometry approaches for precise, multi-scale access to key material properties. The work positions imaging ellipsometry as a powerful platform for monitoring thin-film uniformity, device integrity, and functionality throughout processing, including critical lithographic steps. The study was published in Applied Physics Letters and selected as an Editor’s Pick.