Ayankojo, A.G.; Syritski, V.; Tretjakov, A.; Reut, J.; Boroznjak, R.; Öpik, A.; Rappich, J.; Furchner, A.; Hinrichs, K.: Molecularly Imprinted Polymer Integrated with a Surface Acoustic Wave Technique for Detection of Sulfamethizole. Analytical Chemistry 88 (2016), p. 1476–1484
10.1021/acs.analchem.5b04735
Open Access Version (externer Anbieter)
Abstract:
The synergistic effect of combining molecular imprinting and surface acoustic wave (SAW) technologies for the selective and label-free detection of antibiotics in aqueous environment was demonstrated for the first time. A molecularly imprinted polymer (MIP) for sulfamethizole (SMZ) selective recognition was prepared in a form of a homogeneous thin film on the surface of SAW sensor by oxidative electropolymerization of m-phenylenediamine (mPD) in the presence of SMZ, acting as a template. A special attention was paid to the rational selection of the functional monomer using computational and spectroscopic approaches. SMZ template incorporation and its subsequent release from the polymer was supported by IR microscopic measurements. The thicknesses of SMZ-MIP and non-imprinted reference films were carefully controlled and optimized through electrical charge dosage and ellipsometry measurements. The fabricated SMZ-MIP films were characterized in terms of their binding affinity and selectivity toward the target by analyzing the binding kinetics recorded using SAW system. The SMZ-MIPs had SMZ binding capacity approximately more than eight times higher than the respective non-imprinted polymer (NIP) and were able to discriminate among structurally similar molecules i.e. sulfanilamide and sulfadimethoxine. The presented approach for the facile integration of an antibiotic-sensing layer with SAW technology allowed observing the real-time binding events of the target molecule at relevant sensitivity levels and could be potentially suitable for cost effective fabrication of a multianalyte chemosensor for analysis of hazardous pollutants in aqueous environment.