Polito, R.; Giliberti, V.; Temperini, M.E.; Ritter, E.; Broser, M.; Hegemann, P.; Puskar, L.; Schade, U.; Baldassarre, L.; Ortolani, M.: Light-induced conformational changes of two different Channelrhodopsin mutants probed by difference mid-Infrared microspectroscopy with Synchrotron radiation. In: Andrea Markelz [Ed.] : 2020 45th International Conference on Infrared, Millimeter, and Terahertz Waves (IRMMW-THz) : November 8-13, 2020, a virtual event hosted from Buffalo, New York, USAPiscataway, NJ: IEEE, 2021. - ISBN 978-1-72816-621-6, p. 169-170
10.1109/irmmw-thz46771.2020.9370989
Open Access Version
Abstract:
We apply mid-Infrared (mid-IR) microspectroscopy performed with Synchrotron radiation in order to investigate the functional conformational changes of two different mutants of the light-sensitive transmembrane protein Channelrhodopsin (ChR), a microbial rhodopsin widely used in optogenetic applications. Our results serve as a promising preliminary step towards the investigation of light-sensitive ChR proteins at the nanoscale by means of mid-IR nanospectroscopic techniques based on the use of an atomic force microscope.